Home


How to Reliably Test for GMOs

J €el, M Milavec, D Morisset, D. Plan, G Van den Eede, K Gruden 
Springer  2012  



Softcover  100 pp  ISBN 9781461413899      £45.00
The detection of genetically modified organisms (GMOs) is becoming very complex, with new GMOs, approved and unapproved, constantly entering world markets. Traceability and labelling of GMOs is defined in regulations worldwide, demanding accurate and reliable testing to support the requirements of legislation.

This Brief provides the current state-of-the-art on all key topics involved in GMO testing and is a source of detailed practical information for laboratories. Special focus is given to qualitative and quantitative real-time PCR analysis relevant to all areas where detection and identification rely on nucleic acid-based methods. The following topics, important for testing laboratories, are also discussed: organization of the laboratory, focusing on aspects of the quality system and methods for testing, validation and verification of methods, and measurement uncertainty. The Brief also discusses the new challenges of GMOs and novel modified organisms, using new technologies, and the possible solutions for GMO detection, including bioinformatics tools. Finally, legislation on GMOs and sources of information on GMOs are provided, which are relevant not only to testing laboratories, but to anyone interested in GMOs.

The authors of this Brief have many years of experience in GMO testing, development of real-time PCR methods, implementation of quality system requirements, validations and verification of methods, and measurement uncertainty. The National Institute of Biology is a highly qualified research laboratory and a National Reference Laboratory, which also performs routine analyses of food, feed and seed. The Institute for Health and Consumer Protection of the European Union Joint Research Centre has extensive knowledge and experience of GMO detection. It hosts the European Union Reference Laboratory for GM Food and Feed in addition to chairing the European Network of GMO Laboratories.

Contents

  • Introduction
  • Genetically Modifi ed Organisms
  • Presence of GMOs on the World Market
  • Information on GMOs and Their Detection on the Web
  • Legislation on GMO Labeling and GMO Detection
  • Overview of the Global Situation of GMO Labeling Legislation
  • The European Union Legislation on GMO Labeling
  • and Traceability
  • EU Legislation on GMO Detection
  • Organization of the Laboratory and Quality Management System
  • Introduction
  • Personnel
  • Accommodation and Environmental Conditions
  • Test Methods and Method Validation
  • Measurement Traceability and Reference Materials
  • Sampling
  • Handling of Test Items
  • Assuring Quality of Testing
  • Methods
  • Introduction
  • DNA-Based and Immunological Methods
  • Singleplex, Multiplex Methods
  • Detection Procedure Using DNA-Based Methods
  • GMOs with Stacked Genes
  • Availability of Methods
  • Verifi cation of Methods
  • Introduction
  • Method Validation
  • Aspects of Modular Approach in Method Verifi cation
  • Strategy for Selecting the Methods to Be Implemented
  • Verifi cation Procedure
  • Verifi cation Plan
  • Experimental Verifi cation
  • Data Administration
  • Homogenization and DNA Extraction
  • Introduction
  • Homogenization
  • Risk of Cross-Contamination
  • DNA Extraction
  • DNA Quantifi cation
  • Real-Time PCR
  • Introduction
  • qPCR Equipment
  • Chemistries
  • qPCR Setup, Analysis, and Interpretation
  • Reporting
  • Measurement Uncertainty
  • Introduction
  • MU in GMO Detection
  • Reporting of MU
  • MU and Compliance of Results with Legislation
  • New Challenges
  • Introduction
  • Multiplex qPCR Methods
  • Alternative PCR-Based Technologies
  • Ready-To-Use Multitarget Analytical System
  • Microarrays
  • Digital PCR
  • Sequencing
  • Bioinformatics Support
  • References
  • Index
To find similar publications, click on a keyword below:
DNA : PCR : Recent additions : Springer : analytical methods : biotechnology : food & beverage products : food science : genetically modified organisms : microarrays : sequencing

Terms & Conditions | Privacy Statement

Last Modified 16/12/2013 © CPL Scientific Publishing Services Limited

Search this site Environment Ecology Energy Bioproducts Food Biotechnology Agriculture Biocontrol & IPM Life Sciences Chemistry Business