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Phytophthora: Identifying Species by Morphology and DNA Fingerprints

Mannon E. Gallegly and Chuanxue Hong 
APS Press  2008  



Softcover, sprial bound  168 pp  ISBN 9780890543641      £78.00
The genus Phytophthora, known as the 'plant destroyer', is one of the most important group of disease causing organisms. This genus contains the potato blight pathogen, Phytophthora infestans, and the sudden oak death pathogen, Phytophthora ramorum. Currently the latter species is classified as invasive, and a second one, Phytophthora kernoviae, is about to be placed in this category. Intensive surveys for the presence of these new potentially deadly pathogens have been underway and will continue with funding to search for the second invasive species. Whether or not additional new species with potential destructiveness are found, there are older known species which can erupt at any time and severely damage our crop plants. Correctly identifying them to species level is the first critical step in mitigating crop health risk locally and ensuring plant biosecurity globally.

The new identification key, Phytophthora: Identifying Species by Morphology and DNA Fingerprints, enables diagnosticians and regulatory personnel as well as researchers to identify Phytophthora species w ith speed and confidence. There were only about 50 identified species when the last key was published in 1990, but now that species number is approaching 100. In addition, there have been tremendous efforts in search of a reliable, high-resolution molecular character for rapid identification. This book presents a new key, integrating the classical morphological approach and a new DNA fingerprinting technique called PCR-SSCP. The dichotomous key uses minimal morphological characters, followed by pictorial illustrations. The DNA fingerprint key uses only the rDNA-ITS region amplified with a single pair of primers; a detailed step-by-step fingerprinting protocol is provided.

A total of 652 original photos are included to illustrate individual species covered as well as a partial list of other molecular characters used for description of new species and differentiation of existing species in recent years. This book will be an excellent resource for those who are interested in identifying Phytophthora species. Sixty of the important species and taxons are presented in the book. Also, the framework of the identification keys in the book will readily accept the introduction of additional species as they are studied and described.

  • Each species is presented on its own two-page section
  • Each section includes a description, PCR-SSCP fingerprint key, and illustrations for morphological characters.
  • Integration of classical approach and modern DNA finger printing technology - This enables students, teaches, diagnosticians, and researchers to correctly identify Phytophthora; they can use one method to identify and another to confirm isolate identities. Plus, it allows those who are skilled in DNA fingerprinting techniques to learn the classical approach and visa versa.
  • New dichotomous-like key is presented using minimal morphological characters and the DNA fingerprint key uses only the rDNA-ITS region amplified with a single pair of universal primers - This enables plant health regulatory personnel and diagnosticians to quickly identify Phytophthora species.
  • Pictorial illustrations of morphological characters used in the key and description of individual species - This helps beginners to understand and master essential taxonomic terms and diagnostic characters of individual species. It will also bring them up to speed in their new research and diagnostic service positions.
  • Step by step procedures are provided for all methods used in development of both keys - This allows key users to easily become adept in species identification.
  • Provides a PCR-SSCP fingerprint key along with the protocol of how to use it - This DNA fingerprint technique is quicker and can be used as a confirmation of the morphological identification. Diagnosticians and beginning students can easily become proficient using this method with the straightforward protocol.

Contents

Chapter 1: The Morphological Key
Appendixes: 1. General procedures for morphological identification 2. Illustrations of definitive morphological characters 3. Growth media and methods

Chapter 2: The DNA Fingerprint Key
Appendixes: 1. The PCR-SSCP protocol 2. A partial list of other molecular fingerprinting techniques

Chapter 3: Morphological Characters and DNA Fingerprints of Individual Species
Phytophthora alni Phytophthora boehmeriae Phytophthora botryosa Phytophthora cactorum Phytophthora cajani Phytophthora cambivora Phytophthora capsici Phytophthora cinnamomi var. cinnamomi Phytophthora cinnamomi var. parvispora Phytophthora citricola I Phytophthora citricola II Phytophthora citricola III Phytophthora citrophthora Phytophthora clandestina Phytophthora colocasiae Phytophthora cryptogrea Phytophthora drechsleri Phytophthora erythroseptica Phytophthora europaea Phytophthora fragariae Phytophthora hedraiandra Phytophthora heveae Phytophthora hibernalis Phytophthora humicola Phytophthora idaei Phytophthora ilicis Phytophthora infestans Phytophthora insolita Phytophthora inundata Phytophthora iranica Phytophthora katsurae Phytophthora lateralis Phytophthora meadii Phytophthora medicaginis Phytophthora megakarya Phytophthora megasperma I Phytophthora megasperma II Phytophthora megasperma III Phytophthora melonis Phytophthora mexicana Phytophthora mirabilis Phytophthora nemorosa Phytophthora nicotianae Phytophthora palmivora Phytophthora phaseoli Phytophthora pistaciae Phytophthora porri Phytophthora primulae Phytophthora pseudosyringae Phytophthora pseudotsugae Phytophthora pshychrophila Phytophthora quercina Phytophthora ramorum Phytophthora rubi Phytophthora sojae Phytophthora syringae Phytophthora tentaculata Phytophthora trifolii Phytophthora tropicalis
Appendix: Host, geographic origin, provider, and culture deposits of key isolates

Glossary
Literature Cited
Index

To find similar publications, click on a keyword below:
American Phytopathological Society : DNA : Identification guide : morphology : mycology : plant pathology : plant science

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