Proteomics - Methods and Protocols

Edited by Reinders, Jörg; Sickmann, Albert 
Springer (Humana Press)  2009  

Hardcover  420 pp  ISBN 9781607611561      £90.00
Since the development of the concept of "proteomics," great progress has granted deeper insight into the cellular network, but the enormous range of protein abundance, dynamics, and interactions leaves many further challenges in the field. In Proteomics: Methods and Protocols, experts in proteomic research have contributed detailed techniques, which present an overview of the contemporary challenges and possibilities in the various areas of proteomics, as well as cutting-edge protocols that provide concrete examples for successful proteome analysis. Written in a clear and concise way, authoritative and easy-to-use, this book will serve as a valuable reference book for laboratories around the world and raise interest in the complex and vital field of proteomics research.

Written for: Protein scientists, molecular and cellular biologists, mass spectrometrists


Part I: Introduction

1. Introduction to Proteomics, Friedrich Lottspeich

Part II: Electrophorestic Separations

2. High-Resolution Two-Dimensional Electrophoresis, Walter Weiss and Angelika Görg

3. Non-Classical 2-D Electrophoresis, Jacqueline Burré, Ilka Wittig, and Hermann Schägger

4. Protein Detection and Quantitation Technologies for Gel-Based Proteome Analysis, Walter Weiss, Florian Weiland, and Angelika Görg

Part III: Mass Spectrometry and Tandem Mass Spectrometry Applications

5. MALDI MS, Rainer Cramer

6. Capillary Electrophoresis Coupled to Mass Spectrometry for Proteomic Profiling of Human Urine and Biomarker Discovery , Petra Zürbig, Eric Schiffer, and Harald Mischak

7. A Newcomer€s Guide to Nano-Liquid-Chromatography of Peptides, Thomas Fröhlich and Georg J. Arnold

8. Multidimensional Protein Identification Technology (MudPIT), Katharina Lohrig and Dirk Wolters

9. Characterization of Platelet Proteins Using Peptide Centric Proteomics, Oliver Simon, Stefanie Wortelkamp, and Albert Sickmann

10. Identification of the Molecular Composition of the 20S Proteasome of Mouse Intestine by High Resolution Mass Spectrometric Proteome Analysis, Reinhold Weber, Regina Preywisch, Nikolay Youhnovski, Marcus Groettrup, and Michael Przybylski

Part IV: Quantitative Proteomics

11. Liquid Chromatography-Mass Spectrometry-Based Quantitative Proteomics, Michael W. Linscheid, Robert Ahrends, Stefan Pieper, and Andreas Kühn

12. iTRAQ-Labelling of In-Gel Digested Proteins for Relative Quantification, Carla Schmidt and Henning Urlaub

13. Electrospray Mass Spectrometry for Quantitative Plasma Proteome Analysis, Hong Wang and Sam Hanash

Part V: Interpretation of Mass Spectrometry Data

14. Algorithms and Databases, Lennart Martens and Rolf Apweiler

15. Shotgun Protein Identification and Quantification by Mass Spectrometry, Bingwen Lu, Tao Xu, Robin Park, and John R. Yates III

Part VI: Analysis of Protein Modifications

16. Proteomics Identification of Oxidatively Modified Proteins in Brain, Rukhsana Sultana, Marzia Perluigi, and D. Allan Butterfield

17. Isotope-Labeling and Affinity Enrichment of Phosphopeptides for Proteomic Analysis Using Liquid Chromatography-Tandem Mass Spectrometry, Uma Kota, Ko-yi Chien, and Michael B. Goshe

Part VII: Subcellular Proteomics

18. Organelle Proteomics: Reduction of Sample Complexity by Enzymatic In-Gel Selection of Native Proteins, Veronika Reisinger and Lutz A. Eichacker

19. Isolation of Plasma Membranes from the Nervous System by Countercurrent Distribution in Aqueous Polymer Two-Phase Systems, Jens Schindler and Hans Gerd Nothwang

20. Enrichment and Preparation of Plasma Membrane Proteins from Arabidopsis Thaliana for Global Proteomic Analysis Using Liquid Chromatography-Tandem Mass Spectrometry, Srijeet K. Mitra, Steven D. Clouse, and Michael B. Goshe

Part VIII: Analysis of Protein Interactions

21. Tandem Affinity Purification of Protein Complexes from Mammalian Cells by the Strep/FLAG (SF)-TAP Tag, Christian Johannes Gloeckner, Karsten Boldt, Annette Schumacher, and Marius Ueffing

22. Sequential Peptide Affinity (SPA) Purification System for the Systematic Isolation and Identification of Protein Complexes from Escherichia coli, Mohan Babu, Gareth Butland, Oxana Pogoutse, Joyce Li, Jack F. Greenblatt, and Andrew Emili

23. Bioinformatical Approaches to Detect and Analyze Protein Interactions, Beate Krüger and Thomas Dandekar
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analytical methods : biochemistry : biotechnology : cell biology : methods : proteins : proteomics : protocols

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