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Post-translational Modifications of Proteins - Tools for Functional Proteomics (2nd edition)

Edited by C Kannicht 
Humana Press  May 2008  



Hardcover  390 pp  ISBN 9781588297198      £72.00
The majority of all proteins undergo co- and/or post-translational modifications, crucially altering physical and chemical properties, folding, conformation distribution, stability, activity, and, consequently, the function of the proteins. In Post-translational Modifications of Proteins: Tools for Functional Proteomics, Second Edition, this important area of research is brought up-to-date by the leading scientists in the field. This compilation of detailed protocols focuses on protein function, proteome research and characterization of pharmaceutical proteins, while following the successful format of the Methods in Molecular Biology€ series. Each chapter provides a brief introduction to the topic, step-by-step laboratory protocols, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting edge, Post-translational Modifications of Proteins: Tools for Functional Proteomics, Second Edition serves as practical guide for researchers working in the field of protein structure-function relationships and the rapidly growing field of proteomics, as well as scientists in the pharmaceutical industries.

Written for researchers studying protein structure-function and proteomics, pharmaceutical scientists, cell biologists

Contents

Preface
Contributors

  • Disulfide Bond Mapping by Cyanylation-induced Cleavage and Mass Spectrometry - Jiang Wu
  • Detection of Post-translational Modifi cations by Fluorescent Staining of Two-Dimensional Gels - Archana M. Jacob and Chris W. Turck
  • Identification of Protein Phosphorylation Sites by Advanced LC-ESI-MS/MS Methods - Christoph Weise and Christof Lenz
  • Analysis of Tyrosine-O-Sulfation - Jens R. Bundgaard, Jette W. Sen, Anders H. Johnsen, and Jens F. Rehfeld
  • a-Amidated Peptides: Approaches for Analysis - Gregory P. Mueller and William J. Driscoll
  • g-Glutamate and b-Hydroxyaspartate in Proteins - Francis J. Castellino, Victoria A. Ploplis, and Li Zhang
  • Lysine Hydroxylation and Cross-linking of Collagen - Mitsuo Yamauchi and Masashi Shiiba
  • Mass Spectrometric Determination of Protein Ubiquitination - Carol E. Parker, Maria R. E. Warren, Viorel Mocanu, Susanna F. Greer, and Christoph H. Borchers
  • Analysis of Sumoylation - Andrea Pichler
  • Detection and Analysis of Protein ISGylation - Tomoharu Takeuchi and Hideyoshi Yokosawa
  • Analysis of Methylation, Acetylation, and Other Modifi cations in Bacterial Ribosomal Proteins - Randy J. Arnold, William Running, and James P. Reilly
  • Analysis of S-Acylation of Proteins - Michael Veit, Evgeni Ponimaskin, and Michael F. G. Schmidt
  • Metabolic Labeling and Structural Analysis of Glycosylphosphatidylinositols from Parasitic Protozoa - Nahid Azzouz, Peter Gerold, and Ralph T. Schwarz
  • 2-Dimensional Electrophoresis: Detection of Glycosylation and Infl uence on Spot Pattern - Klemens Löster and Christoph Kannicht
  • Carbohydrate Composition Analysis of Glycoproteins by HPLC Using Highly Fluorescent Anthranilic Acid (AA) Tag - George N. Saddic, Shirish T. Dhume, and Kalyan R. Anumula
  • Enzymatical Hydrolysis of N-Glycans from Glycoproteins and Fluorescent Labeling by 2-Aminobenzamide (2-AB) - Rolf Nuck
  • Separation of N-Glycans by HPLC - Martin Gohlke and Véronique Blanchard
  • Enzymatic Sequence Analysis of N-Glycans by Exoglycosidase Cleavage and Mass Spectrometry€detection of Lewis X Structures - Christoph Kannicht, Detlef Grunow, and Lothar Lucka
  • Immunochemical Methods for the Rapid Screening of the O-Glycosidically Linked N-Acetylglucosamine Modifi cation of Proteins - Michael Ahrend, Angela Käberich, Marie-Therese Fergen, and Brigitte Schmitz
  • Analysis of O-Glycosylation - Juan J. Calvete and Libia Sanz
  • Characterization of Site-specifi c N-Glycosylation - Katalin F. Medzihradszky
  • Monitoring Glycosylation of Therapeutic Glycoproteins for Consistency by HPLC Using Highly Fluorescent Anthranilic Acid (AA) Tag - Shirish T. Dhume, George N. Saddic, and Kalyan R. Anumula
  • Comparability and Monitoring Immunogenic N-linked Oligosaccharides from Recombinant Monoclonal Antibodies from Two Different Cell Lines using HPLC with Fluorescence Detection and Mass Spectrometry - Bruce R. Kilgore, Adam W. Lucka, Rekha Patel, Bruce A. Andrien, Jr., and Shirish T. Dhume
  • Mass Spectrometry and HPLC with Fluorescent Detection-Based Orthogonal Approaches to Characterize N-linked Oligosaccharides of Recombinant Monoclonal Antibodies - Adam W. Lucka, Bruce R. Kilgore, Rekha Patel, Bruce A. Andrien, Jr., and Shirish T. Dhume
  • Web-based Computational Tools for the Prediction and Analysis of Post-translational Modifications of Proteins - Vladimir A. Ivanisenko, Dmitry A. Afonnikov, and Nikolay A. Kolchanov

Index

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Humana Press : biochemistry : biotechnology : cell biology : proteins : proteomics

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