Plant Signal Transduction - Methods and Protocols

Edited by T Pfannschmidt 
Springer  October 2008  

Hardcover  357 pp  ISBN 9781588299437      £67.00
During recent years, research has greatly expanded our understanding of the sophisticated molecular network of responses which enable plants to develop, survive and propagate under a wide range of conditions. In Plant Signal Transduction: Methods and Protocols, an international panel of experts provide well-established methods vital to analyzing plant signal transduction on the molecular level. Featuring experimental procedures on several of the most popular model organisms, the volume focuses on in planta analyses and the proteins involved in signal transduction in order to aid with the establishment of laboratory techniques or the modification of the protocols for other plants.

As part of the highly successful Methods in Molecular Biology€ series, the chapters include brief introductions to the subject, lists of necessary materials, readily reproducible laboratory protocols, and tips on trouble-shooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Plant Signal Transduction: Methods and Protocols will benefit plant scientists wishing to improve their experimental approaches and delve further into this exciting and important field of study.

Written for plant physiologists, biochemists, geneticists, molecular biologists, and ecosystem researchers


  • Multiple Dimensions in Plant Signal Transduction: An Overview, Jorge J. Casal
  • Phenotyping of Arabidopsis Mutants for Developmental Effects of Gene Deletions, Cordelia Bolle
  • Phenotyping of Abiotic Responses and Hormone Treatments in Arabidopsis, Cordelia Bolle
  • Accurate Real-time Reverse Transcription Quantitative PCR, Marco Klatte and Petra Bauer
  • Probing Spatio-temporal Intracellular Calcium Variations in Plants, Axel Mithöfer, Christian Mazars and Massimo E. Maffei
  • Dynamic Redox Measurements with Redox-Sensitive GFP in Plants by Confocal Laser Scanning Microscopy, Andreas J. Meyer and Thorsten Brach
  • Imaging of Reactive Oxygen Species in vivo, Steven M. Driever, Michael J. Fryer, Philip M. Mullineaux and Neil R. Baker
  • Identification of Thioredoxin Targeted Proteins Using Thioredoxin Single Cysteine Mutant-immobilized Resin, Ken Motohashi, Patrick G. N. Romano, and Toru Hisabori
  • Determination of in vivo Protein Phosphorylation in Photosynthetic Membranes, Julia P. Vainonen, Alexander V. Vener and Eva-Mari Aro
  • Examining Protein Stability and Its Relevance for Plant Growth and Development , Claus Schwechheimer, Björn C. Willige, Melina Zourelidou and Esther M. N. Dohmann,
  • Probing Circadian Rhythms in Chlamydomonas rheinhardtii by Functional Proteomics, Volker Wagner and Maria Mittag
  • Bimolecular Fluorescence Complementation (BiFC) to Study Protein-protein Interactions in Living Plant Cells, Katia Schütze, Klaus Harter and Christina Chaban
  • Fluorescence Cross-Correlation Spectroscopy of Plant Proteins, Hideki Muto, Masataka Kinjo and Kotaro T. Yamamoto
  • The Determination of Protein-protein Interactions by the Mating-based Split-ubiquitin-system (mbSUS), Christopher Grefen, Petr Obrdlik and Klaus Harter
  • Functional Complementation of Yeast Mutants to Study Plant Signalling Pathways, Norbert Mehlmer, Elisabeth Scheikl-Pourkhalil and Markus Teige
  • Phosphatase Activities Analyzed by in vivo Expressions, Alois Schweighofer, Zahra Ayatollahi and Irute Meskiene
  • Chromatin Immunoprecipitation Experiments to Investigate in vivo Binding of Arabidopsis Transcription Factors to Target Sequences, Benjamin Fode and Christiane Gatz
  • Fluorescence-based Electrophoretic Mobility Shift Assay in the Analysis of DNA-binding Proteins, Sebastian Steiner and Thomas Pfannschmidt
  • Analysis of Plant Regulatory DNA sequences by the Yeast-One-Hybrid Assay, Dierk Wanke and Klaus Harter
  • Analysis of Plant Regulatory DNA Sequences by Transient Protoplast Assays and Computer Aided Sequence Evaluation, Kenneth W. Berendzen, Klaus Harter and Dierk Wanke
  • RNA-protein Interaction Mediating Post-transcriptional Regulation in the Circadian System, Jan C. Schöning and Dorothee Staiger
To find similar publications, click on a keyword below:
Springer : analytical methods : methods : plant physiology : plant science : protocols : signalling, plant

Terms & Conditions | Privacy Statement

Last Modified 16/12/2013 © CPL Scientific Publishing Services Limited

Search this site Environment Ecology Energy Bioproducts Food Biotechnology Agriculture Biocontrol & IPM Life Sciences Chemistry Business