Protocols for Micropropagation of Woody Trees and Fruits
Edited by Jain, S.Mohan; Häggman, H.
Micropropagation has become a reliable and routine approach for large-scale rapid plant multiplication, which
is based on plant cell, tissue and organ culture on well defined tissue culture media under aseptic conditions.
A lot of research efforts are being made to develop and refine micropropagation methods and culture media
for large-scale plant multiplication of several number of plant species. However, many forest and fruit tree
species still remain recalcitrant to in vitro culture and require highly specific culture conditions for plant growth
Hardcover 562 pp ISBN 9781402063510
The recent challenges on plant cell cycle regulation and the presented potential molecular mechanisms
of recalcitrance are providing excellent background for understanding on totipotency and what is more
development of micropropagation protocols. For large-scale in vitro plant production the important attributes
are the quality, cost effectiveness, maintenance of genetic fidelity, and long-term storage. The need for
appropriate in vitro plant regeneration methods for woody plants, including both forest and fruit trees,
is still overwhelming in order to overcome problems facing micropropagation such as somaclonal variation,
recalcitrant rooting, hyperhydricity, polyphenols, loss of material during hardening and quality of plant material.
Moreover, micropropagation may be utilized, in basic research, in production of virus-free planting material,
cryopreservation of endangered and elite woody species, applications in tree breeding and reforestation.
Written for: Students, researchers, horticulturists, forest geneticists, biotech companies
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: plant propagation
: plant science
: trees and timber