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Organelle Proteomics

Edited by Pflieger, Delphine; Rossier, Jean 
Springer  March 2008  



Softcover  422 pp  ISBN 9781617377723      £72.00
Proteomic analyses have proven to be an invaluable tool in obtaining high-throughput protein identification from low-abundance, complex biological samples. In Organelle Proteomics, detailed protocols provide step-by-step instructions to successfully study organelle proteomes by performing the purification of the various organelles present in eukaryotic cells, as well as by preparing certain sub-fractions of organelles.

A series of chapters cover the whole analytical procedure of organelle characterization, from its purification starting with whole cells up to protein identification using mass spectrometry. Devoted to methods enabling a global estimate of the reliability of the protein list assigned to an organelle, Organelle Proteomics allows scientists to gain a vital and important understanding of organelle study. Of interest to molecular and cellular biologists, biochemists, and proteomics specialists

Contents

  • Analysis of organelles by on-line two-dimensional liquid chromatography - tandem mass spectrometry (2DLC-MS/MS) Edwin P. Romijn and John R. Yates III.
  • Purification and proteomic analysis of chloroplasts and their sub-organellar compartments Daniel Salvi, Norbert Rolland, Jacques Joyard and Myriam Ferro
  • Proteomic analysis of the stacked Golgi complex Mark S. Lowenthal, Kathryn E. Howell, and Christine C. Wu
  • Purification of Saccharomyces cerevisiae mitochondria by zone-electrophoresis in a free flow device (ZE-FFE) Hans Zischka, Norbert Kinkl, Ralf J. Braun and Marius Ueffing
  • Preparation of Respiratory Chain Complexes from Saccharomyces cerevisiae Wild Type and Mutant Mitochondria : Activity Measurement and Subunit Composition Analysis Claire Lemaire and Geneviève Dujardin
  • Mitochondrial proteomics: Analysis of a whole mitochondrial extract with two-dimensional electrophoresis Thierry Rabilloud.
  • Purification and proteomic analysis of the mouse liver mitochondrial inner membrane Sandrine Da Cruz and Jean-Claude Martinou
  • Subcellular Fractionation and Proteomics of Nuclear Envelopes Laurence Florens, Nadia Korfali and Eric C. Schirmer.
  • Purification and proteomic analysis of the nuclear insoluble protein fraction Tsuneyoshi Horigome, Kazuhiro Furukawa and Kohei Ishii
  • Preparation Methods of Human Metaphase Chromosomes for Their Proteome Analysis. Kiichi Fukui and Hideaki Takata
  • Purification and proteomic analysis of plant plasma membranes Erik Alexandersson, Niklas Gustavsson, Katja Bernfur, Adine Karlsson, Per Kjellbom and Christer Larsson
  • Protocol to enrich and analyze plasma membrane proteins from frozen tissue Jacek R. Wisniewski
  • Proteomic characterization of membrane protein topology Adele R. Blackler and Christine C. Wu
  • Free Flow Isoelectric Focussing (FF-IEF): A method for the separation of both hydrophilic and hydrophobic proteins of rat liver peroxisomes Markus Islinger and Gerhard Weber
  • Use of gas-phase-fractionation to increase protein identifications: application to the peroxisome Jacob Kennedy and Eugene C. Yi
  • Purification and proteomic analysis of lysosomal integral membrane proteins Huiwen Zhang, Xiaolian Fan, Rick Bagshaw, Don J. Mahuran, John W. Callahan.
  • Affinity purification of soluble lysosomal proteins for mass spectrometric identification Sylvie Kieffer-Jaquinod, Agnès Chapel, Jérôme Garin and Agnès Journet
  • Purification and proteomic analysis of synaptic vesicles Holly D. Cox and Charles M. Thompson.
  • Purification and proteomic analysis of pancreatic zymogen granule membranes Xuequn Chen and Philip C. Andrews
  • Isolation and proteomic analysis of Chlamydomonas centrioles Lani C. Keller and Wallace F. Marshall.
  • Purification and proteomic analysis of 20S proteasomes from human cells Marie-Pierre Bousquet-Dubouch, Sandrine Uttenweiler-Joseph, Manuelle Ducoux-Petit, Mariette Matondo, Bernard Monsarrat, and Odile Burlet-Schiltz.
  • Characterization of E. coli ribosomal particles: combined analysis of whole proteins by mass spectrometry and of proteolytic digests by liquid chromatography - tandem mass spectrometry. Isabelle Iost, Julie Charollais, Joëlle Vinh and Delphine Pflieger
  • Assessment of organelle purity using antibodies and specific assays: the example of the chloroplast envelope Daniel Salvi, Norbert Rolland, Jacques Joyard and Myriam Ferro
  • Identifying bona fide components of an organelle by isotope coded labelling of subcellular fractions: an example in peroxisomes Marcello Marelli, Alexey I. Nesvizhskii and John D. Aitchison
  • Determination of Genuine Residents of Plant Endomembrane Organelles using Isotope Tagging and Multivariate Statistics Kathryn S. Lilley and Tom P.J. Dunkley
  • Quantitative Proteomic Analysis to Profile Dynamic Changes in the Spatial Distribution of Cellular Proteins Wei Yan, Daehee Hwang, Ruedi Aebersold
  • Use of transcriptomic data to support organelle proteomic analysis Wallace F. Marshall
To find similar publications, click on a keyword below:
Springer : analytical methods : biochemistry : cell biology : mitochondria : molecular biology : plant physiology : plant science : plastids : proteomics

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