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Baculovirus and Insect Cell Expression Protocols - 2nd edition

Edited by Murhammer, David W. 
Humana Press  2007  



Hardcover  470 pp  ISBN 9781588295378      £135.00
This second edition is a detailed guide for using the baculovirus expression vector system (BEVS) and/or insect cells to produce recombinant proteins. Procedures for the development and production of baculovirus insecticides at both laboratory and large scale are described in the kind of step-by-step format that the Methods in Molecular Biology series helped standardize.

This book is a one-stop source for information on baculoviruses, and includes sections on using modified baculoviruses to express genes in mammalian cells, using Drosophila cell lines, isolating new cell lines, and developing serum-free medium. Specific applications, including using baculovirus and insect cell system to study apoptosis, are also discussed.

This updated and expanded edition provides a perfect lab manual for biochemists, molecular biologists, biochemical engineers, and others for using the BEVS or insect cell lines to produce recombinant proteins.

Of interest to: Biochemists, molecular and cell biologists, biochemical engineers

Contents

PART I. INTRODUCTION

Chapter 1 Useful Tips, Widely Used Techniques and Quantifying Cell Metabolic Behavior - David W. Murhammer

PART II. BACULOVIRUS MOLECULAR BIOLOGY/DEVELOPMENT OF RECOMBINANT BACULOVIRUSES

Chapter 2 Introduction to Baculovirus Molecular Biology - Barbara J. Kelly, Linda A. King, and Robert D. Possee

Chapter 3 Baculovirus Transfer Vectors - Robert D. Possee and Linda A. King

Chapter 4 Recombinant Baculovirus Isolation - Linda A. King, Richard Hitchman, and Robert D. Possee

Chapter 5 Gene Expression in Mammalian Cells Using BacMam, a Modified Baculovirus System - James A. Fornwald, Quinn Lu, Dayuan Wang, and Robert S. Ames

PART III. INSECT CELL CULTURE

Chapter 6 Available Lepidopteran Insect Cell Lines - Dwight E. Lynn

Chapter 7 Lepidopteran Insect Cell Line Isolation From Insect Tissue - Dwight E. Lynn

Chapter 8 Development of Serum-Free Media for Lepidopteran Insect Cell Lines - Sprios N. Agathos

Chapter 9 Routine Maintenance and Storage of Lepidopteran Insect Cell Lines and Baculoviruses - Dwight E. Lynn

PART IV. PROTEIN PRODUCTION WITH RECOMBINANT BACULOVIRUSES

Chapter 10 Small Scale Protein Production With the Baculovirus Expression Vector System - Steve Harwood

Chapter 11 Recombinant Protein Production in Large Scale Agitated Bioreactors Using the Baculovirus Expression Vector System - Cynthia B. Elias, Barbara Jardin, and Amine Kamen

Chapter 12 Production of Baculovirus-Expressed Recombinant Proteins in Wave Bioreactors - Sue H. Kadwell and Philip I. Hardwicke

Chapter 13 Protein Production With Recombinant Baculoviruses in Lepidopteran Larvae - Yi Liu, Nathan DeCarolis, and Nikolai van Beek

Chapter 14 Virus-Like Particle and Viral Vector Production Using the BEVS/Insect Cell System: Adeno-Associated Virus Based Products - Marc G. Aucoin, Danielle Jacob, Parminder S. Chahal, Jamal Meghrous, Alice Bernier, and Amine A. Kamen

PART V. RECOMBINANT PROTEIN PRODUCTION WITH TRANSFORMED INSECT CELLS

Chapter 15 Transforming Lepidopteran Insect Cells for Continuous Recombinant Protein Expression - Robert L. Harrison and Donald L Jarvis

Chapter 16 Transformation of Drosophila Cell Lines: An Alternative Approach to Exogenous Protein Expression - Lucy Cherbas and Peter Cherbas

Chapter 17 Transforming Lepidopteran Insect Cells for Improved Protein Processing - Robert L. Harrison and Donald L Jarvis

PART VI. BACULOVIRUS DEVELOPMENT AND PRODUCTION FOR USE AS INSECTICIDES

Chapter 18 Introduction to the Use of Baculoviruses as Biological Insecticides - Bryony C. Bonning and Tyasning Nusawardani

Chapter 19 Baculovirus Insecticide Production in Insect Larvae - Nikolai van Beek and David C. Davis

Chapter 20 Evaluation of the Insecticidal Efficacy of Wild Type and Recombinant Baculoviruses - Huarong Li and Bryony C. Bonning

PART VII. MISCELLANEOUS TECHNIQUES AND APPLICATIONS OF THE BACULOVIRUS/INSECT CELL SYSTEM

Chapter 21 Monitoring and Visualization of Baculovirus Infection Using Green Fluorescent Protein Strategy - Hyung Joon Cha and William E. Bentley

Chapter 22 Alternative Molecular and Reactor Strategies Probing Infection and Production - Yu-Chen Hu and William E. Bentley

Chapter 23 Methods For Gene Silencing with RNAi - John C. March and William E. Bentley

Chapter 24 Use of the Baculovirus/Insect Cell System to Study Apoptosis - Nor Chejanovsky

Chapter 25 Generation of Envelope-Modified Baculoviruses for Gene Delivery Into Mammalian Cells - Christian Hofmann

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Springer : biotechnology : cell biology : entomology : genetics : pest control : proteins : virology

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