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Proteins at Solid-Liquid Interfaces

Edited by Philippe Déjardin 
Springer  2006  



Hardcover  330 p.  ISBN 9783540326571      £157.00
This book opens with a description of fundamental aspects of protein adsorption to surfaces, a phenomenon that plays a key role in biotechnological applications, especially at solid-liquid interfaces. Presented here are methods for studying adsorption kinetics and conformational changes such as optical waveguide lightmode spectroscopy (OWLS). Also described are sensitive bench techniques for measuring the orientation and structure of proteins at solid-liquid interfaces, including total internal reflection ellipsometry (TIRE), dual polarisation interferometry (DPI) and time of flight - secondary ion mass spectrometry (TOF-SIMS).

A model study of fibronectin at polymer surfaces is included, as are studies using microporous membranes and textiles with immobilized enzymes for large-scale applications. Biocompatibility, anti-fouling properties and surface modification to modulate the adsorption and activity of biomolecules are among the other topics addressed in this invaluable book.

Of interest to scientists and graduate students in physical chemistry, biophysics, biochemistry and biotechnology; research engineers.

Contents

Part I Analysis of the Adsorption Kinetics

  • Protein Adsorption Kinetics: Influence of Substrate Electric Potential 1
  • FromKinetics to Structure:HighResolutionMolecular Microscopy, Jeremy J. Ramsden
  • Initial Adsorption Kinetics in a Rectangular Thin Channel, and Coverage-Dependent Structural Transition Observed by Streaming Potential, Philippe Déjardin, Elena N. Vasina

Part II Analysis of the Structure at the Interface

  • Dual Polarisation Interferometry: An Optical Technique toMeasure the Orientation and Structure of Proteins at the Solid-Liquid Interface in Real Time , Neville Freeman
  • Total Internal Reflection Ellipsometry: Monitoring of Proteins on ThinMetal Films 105, Michal Poksinski, Hans Arwin
  • Conformations of Proteins Adsorbed at Liquid-Solid Interfaces, Sylvie Noinville, Madeleine Revault
  • Evaluation of Proteins on Bio-Devices, Satoka Aoyagi, Masahiro Kudo

Part III Some Applications

  • Fibronectin at Polymer Surfaces with Graduated Characteristics, Tilo Pompe, Lars Renner, CarstenWerner
  • Development of Chemical Microreactors by Enzyme Immobilization onto Textiles, Christophe Innocent, Patrick Seta
  • Approaches to Protein Resistance on the Polyacrylonitrile-based Membrane Surface: an Overview, Ling-Shu Wan, Zhi-Kang Xu, Xiao-Jun Huang
  • Modulation of the Adsorption and Activity of Protein/Enzyme on the PolypropyleneMicroporousMembrane Surface by SurfaceModification, Qian Yang, Zhi-Kang Xu, Zheng-Wei Dai
  • Nonbiofouling Surfaces Generated fromPhosphorylcholine-Bearing Polymers, Yasuhiko Iwasaki,Nobuo Nakabayashi,Kazuhiko Ishihara

Subject Index

To find similar publications, click on a keyword below:
Springer : biochemistry : biophysics : biotechnology : chemistry : enzymology : mass spectrometry : proteins : spectroscopy

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