Methods in Gut Microbial Ecology for Ruminants
Edited by Makkar, Harinder P.S.; McSweeney, Christopher S.
Until recently, knowledge of ruminant gut microbiology was primarily obtained using classical culture
based techniques, which probably only account for 10 to 20% of the rumen microbial population.
New gene-based technologies can now be employed to examine microbial diversity through the use of
small sub-unit ribosomal DNA analysis (e.g. 16S rDNA) and to understand the function of complex
microbial ecosystems in the rumen through metagenomic analysis.
Hardcover 225 pp ISBN 9781402037900
These technologies have the potential to revolutionize the understanding of rumen function and will
overcome the limitations of classical based techniques, including isolation and taxonomic identification
of strains important to efficient rumen function and better understanding of the roles of microorganisms
in relation to achieving high productivity and decreasing environmental pollutants. This book presents
a comprehensive up-to-date account of the methodologies and protocols for conventional and
modern molecular techniques that are currently in use for studying the gut microbial ecology of ruminants.
Each chapter has been contributed by experts in the field and methods have been presented in a
recipe-like format designed for direct practical use in the laboratory and also to provide insight into
the most appropriate techniques, their applications and the type of information that could be expected.
The techniques and procedures described are also relevant and adaptable to other gastrointestinal ecosystems
and the microbiology of anaerobic environments in general. This manual will €demystify€ the methods in molecular
microbial ecology for readers who are novice in the field but are excited by the prospects of the technology. It
would also be invaluable for the experienced workers striving for giving new dimension to their research € expanding
the work in other fields and initiating cross-cutting activities.
Of interest to researchers, teachers and students in the areas of biotechnology, molecular biology,
rumen microbiology, rumen ecology and environment ecology.
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