Laboratory Exercises in Microbiology, 3rd ed
Robert A. Pollack
Now in its third edition, Laboratory Exercises in Microbiology contains a variety of interactive
activities and experiments that teach readers the basic concepts of microbiology. It covers methods
that allow the safe movement or transfer of microbial cells from one type of growth environment,
classification and identification of microbes, microbial biochemistry, and medical, food, and
environmental microbiology. The labs are introduced in a clear and concise manner, while maintaining a
Paperback 304 ISBN 9780470133927
Written specifically for allied health students, this book focuses on what students need to succeed in the lab: clear,
concise exercises and activities, rather than content that is simply adapted from a standard microbiology textbook.
Throughout the manual, a variety of interactive activities and experiments convey the basic concepts
of microbiology in a student-friendly way. Students also have the opportunity to explore methods
that allow the safe movement or transfer of microbial cells from one type of growth environment to another,
classification and identification of microbes, microbial biochemistry, as well as laboratory exercises from medical,
food, and environmental microbiology.
Introduction: Laboratory Operations and Safety:
PART I. GENERAL MICROSCOPY AND ASEPTIC TECHNIQUE.
1. Laboratory Safety: Introduction to the Microscope.
2. Transfer and Isolation Techniques.
Microbes in the Environment.
PART II. MICROBIAL MORPHOLOGY, DIFFERENTIAL STAINS.
3. Cultural and Cellular Morphology.
4. Bacterial Growth.
5. Gram Stain.
6. Endospore Stain.
Hanging Drop Technique.
8. Viruses - Visualization and Enumeration.
PART III. MICROBIAL CONTROL AND BIOCHEMISTRY.
10. Microbial Sensitivity Testing:
Ultraviolet Light Sensitivity.
11. Bacterial Biochemistry.
12. Gas Requirements of Microorganisms.
13. Specialized Media.
PART IV. MEDICAL MICROBIOLOGY.
16. Speciman-Handling Protocols.
17. Specific Laboratory Tests.
19. Identification of Enteric Pathogens: Traditional Methods.
20. Identification of Enteric Pathogens: Rapid Identification Methods.
21. Identification of a Bacterial Unknown: The Gram-Negative Unknown.
22. Identification of a Bacterial Unknown: The Gram-Positive Cocci.
PART V. FOOD AND ENVIRONMENTAL MICROBIOLOGY.
23. Identification and Quantification of Microbial Numbers in a Water Sample.
24. Identification of Microbes in Beef and Poultry and the Quantification of Microbial Numbers.
25. Soil Microbiology.
26. Microbial Ecology.
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