RNAi: A Guide to Gene Silencing

Edited by Gregory J. Hannon 
CSHL  2003  

Paperback  436 pp  ISBN 9780879697044      £52.00
The ability of double stranded RNA to inhibit the expression of genes with an appropriate sequence can be harnessed to silence target genes in vitro in a new and powerful way. This volume combines reliable RNAi protocols for a variety of species with discussion of strategies for the effective design of experiments using this important new technique that is changing the way experimental science is done.


  • Chapter 1. Sense Cosuppression in Plants: Past, Present, and Future Richard A. Jorgensen
  • Chapter 2. Transgene Cosuppression in Animals James A. Birchler, Manika Pal-Bhadra, and Utpal Bhadra
  • Chapter 3. Regulation of the Genome by Double€stranded RNA Marjori Matzke, M. Florian Mette, Tatsuo Kanno, Werner Aufsatz, and Antonius J.M. Matzke
  • Chapter 4. RNAi in Caenorhabditis elegans René F. Ketting, Marcel Tijsterman, and Ronald H.A. Plasterk
  • Chapter 5. Small RNAs, Big Biology: Biochemical Studies of RNA Interference Gregory J. Hannon and Phillip D. Zamore
  • Chapter 6.shRNA-mediated Silencing of Mammalian Gene Expression Michael T. McManus and Douglas S. Conklin
  • Chapter 7. Transposable Elements, RNA Interference, and the Origin of Heterochromatin Rob Martienssen, Tom Volpe, Zach Lippman, Ann-Valerie Gendrel, Catherine Kidner, Pablo Rabinowicz, and Vincent Colot
  • Chapter 8. The Ribonuclease III Superfamily: Forms and Functions in RNA Maturation, Decay, and Gene Silencing Allen W. Nicholson
  • Chapter 9. RNA-dependent RNA Polymerase in Gene Silencing Luyun Huang, John Gledhill, and Craig E. Cameron
  • Chapter 10. Structure and Function of Heterochromatin: Implications for Epigenetic Gene Silencing and Genome Organization Ira M. Hall and Shiv I.S. Grewal
  • Chapter 11. Total Interference: Genome-wide RNAi Screens in C. elegans Andrew G. Fraser and Julie Ahringer
  • Chapter 12. PTGS Approaches to Large Scale Functional Genomics in Plants Tessa M. Burch€Smith, Jennifer L. Miller, and S.P. Dinesh-Kumar
  • Chapter 13. Mammalian RNA Interference Thomas Tuschl
  • Chapter 14. RNAi in Avian Embryos Esther T. Stoeckli
  • Chapter 15. Guide to RNAi in Mouse Oocytes and Preimplantation Embryos Paula Stein and Petr Svoboda
  • Chapter 16. RNAi Technologies in Drosophila Cell Culture Scott M. Hammond
  • Chapter 17. RNAi Applications in Drosophila melanogaster Richard W. Carthew
  • Chapter 18. RNA Interference in Trypanosoma brucei and Other Nonclassical Model Organisms Elisabetta Ullu and Christian Tschudi
To find similar publications, click on a keyword below:
Cold Spring Harbor : RNA : animal science : genes : methods : molecular biology : nucleic acids : plant science

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