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Plant Cytogenetics, Second Edition

Ram J Singh 
CRC Press  December 2002  



Hardback  488pp  ISBN 9780849323881      £107.00
  • New chapters on Extra Chromosomal Inheritance and the Mode of Reproduction (apomixis) in Plants
  • New sections include the Molecular Basis of Heredity, Genome In Situ Hybridizations, and the Molecular Method of Genome Analysiso Includes an extensive glossary to facilitate ease of use
  • An elaboration on the cytogenetic basis of somaclonal variation generation through cell and tissue culture

Plant cytogenetics has progressed at a rapid rate since the publication of the first edition. Plant Cytogenetics, Second Edition presents an up-to-date review of cytogenetics. It covers the latest in the various classical and modern techniques in the handling of chromosomes, karyotype analysis, genetics of meiosis, genomic relationships, and chromosome manipulation. It includes new chapters on extra chromosomal inheritance and the mode of reproduction in plants, paricularly apomixis, as well as new sections on the molecular basis of heredity, genomic in situ hybridization, and the classical and molecular methods of genome analysis. The author also elaborates on the cytogenetic basis of somaclonal variation generated through cell and tissue culture.

Contents

THE HANDLING OF PLANT CHROMOSOMES
Introduction
Squash Technique - Mitotic and Meiotic Chromosomes
Smear Technique for Plant Chromosomes
Pollen Staining
Pollen-Stigma Incompatibility
Fluorescence in situ Hybridization (FISH)
Total DNA Extraction-Plant Genomic DNA
Determination of Nuclear DNA Content of Plants by Flow Cytometry
Karyotyping and Sorting of Plant Chromosomes by Flow Cytometry
Production of Wide Hybrids Through In Vitro Technique

CELL DIVISION
Introduction
Mitosis
Meiosis

GENETIC CONTROL OF MEIOSIS
Introduction
Synaptic Mutants
Genetic Control of Recombination
Genes Responsible for Chromosome Disjunction
Other Meiotic Mutants
Role of Heterochromatin in Chromosome Pairing
Diploid-like Meiosis in Allopolyploids
Haploidy
Male Sterility

MODE OF REPRODUCTION IN PLANTS
Introduction
Sexual Reproduction
Asexual Reproduction
The Chromosomal Basis of Sex Determination in Plants

KARYOTYPE ANALYSIS
Introduction
Nomenclature of Chromosomes
Karyotype Analysis by Mitotic Metaphase Chromosomes
Karyotype Analysis by Pachytene Chromosomes
Karyotype Analysis by Flow Cytometry
Karyotype Analysis by Image Analysis
Chromosome Number of Economically Important Plants

CHROMOSOMAL ABERRATIONS - STRUCTURAL CHROMOSOME CHANGES
Deficiencies
Duplications
Interchanges
Inversions

CHROMOSOMAL ABERRATIONS - NUMERICAL CHROMOSOME CHANGES
Euploidy
Aneuploidy: Trisomics
Monosomics and Nullisomics
Alien Addition Lines
Alien Substitution Lines

GENOME ANALYSIS
Introduction
Classical Taxonomy and Genome Designations
Genome Relationships among Diploid Species
Genomes of Glycine
Genomes of Gossypium
Ancestors of Allopolyploid Species
Genome Analysis by Molecular Methods

CHROMOSOMAL ABERRATIONS IN CELL AND TISSUE CULTURE
Introduction
Chromosomal Aberrations in Callus
Mechanism of Chromosomal Aberrations in Culture
Chromosomal Aberrations in Regenerated Plants
Chromosomal Aberrations in Haploid Callus and Their Regenerants
Chromosomal Aberrations in Somatic Hybrids

TRANSGENIC CROPS
Introduction
Present Status of Transgenic Crops
Production of Transgenic Crops
Chromosome Aberrations in Transgenic Crops
Cytological Basis of Gene Silencing

APPENDICES

REFERENCES

GLOSSARY

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CRC Press : biotechnology : cell culture : genetics : molecular biology : plant science

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