Methods for Affinity-Based Separations of Enzymes and Proteins
Edited by Gupta, M. N.
Hardcover X, 225 pp ISBN 9783764363062
One major concern of biotechnology is either using enzymes or producing them.
Enzyme/protein production is therefore an important starting point for biotechnology.
Bioseparation or Downstream Processing constitutes about 40-90% of the total production
cost. Driven by economics, highly selective technologies applicable to large-scale
processing have emerged during the last decade. These technologies are slowly diffusing to
enzymologists who are working on a smaller scale, looking for fast and efficient purification
protocols. The affinity-based techniques (including precipitation, two-phase extractions,
expanded bed chromatography, perfusion chromatography and monoliths) described in
this volume provide current and new cutting-edge methods. Consequently, the book is of
main interest to researchers in biochemistry, biochemical engineering and biotechnology,
working either in academic or industrial sectors.
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